Because ettan dige system comprises many different products, references to the appropriate manuals for further details are made when necessary, see table 11. Experience the power of 2d electrophoresis with 2d dige. Aa1001 p1 ettan daltsix electrophoresis system for large format and medium throughput 2d electrophoresis. Ettan ipgphor 3 instrument user manual 11003458 edition ab 9 1.
In each compartment, electrodes produce the electric field to cause. Ettan ipgphor 3 with ettan ipgphor manifold offers a. Ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, speed, reproducibility and high proteinloading capacity. View and download ipecs es3052g user manual online. Ettan ipgphor 3 isoelectric focusing system, ge healthcare.
Software overview 3 ettan ipgphor 3 control software user manual 11003459 edition ab 11 3 software overview 3. Ettan ipgphor system ge healthcare according to the user manual. Ettan dige system users manual university of alberta. Ge healthcare manifold kit for ettan ipgphor 3 isoelectric. The gels were stored overnight at 4c before scanning in typhoon fla 9000 biomolecular imager.
Ipgphor manifold light 45002621 routine applications. The ettan ipgphor cup loading manifold user manual contains instructions for firstdimension isoelectric focusing of proteins on ipg strips. Connect only to coolant sources with regulated pressure. Two glass plates are joined along one edge by a hinge strip of silicone rubber, and the vinyl side spacers 1. Ettan ipgphor 3 isoelectric focusing unit 45002623 unit to run first dimension ief ipgphor manifold, complete 450026 for the most stringent applications. Processing small tissue or cell samples using sample grinding kit. Protease inhibition using protease inhibitor mix sigmaaldrich. Perform a bradford protein assay biorad protein assay instructions come with. Ettan ipgphor 3 with ettan ipgphor manifold offers a dedicated platform for running up to 12 firstdimension ief separations with immobiline drystrip gels. Pdf proteomic profiling of the epithelialmesenchymal. The default installation of the ettan ipgphor ii control software will be on the local c drive within the folder titled amersham biosciences.
Isoelectric focusing was done using an ipgphor 3 apparatus ge at 20c under drystrip cover fluid with the following voltages and times. Ethernetip secure communic ation module user manual, publication enetum003 provides information on setting up authentication, encryption, and firewalls, typical architectures, and diagnostics for modules equipped with secure communication functionality. Software overview 3 ettan ipgphor 3 control software user manual 11003459 edition ab 15 3. The software can be used to control up to four ettan ipgphor 3 ief units simultaneously, each running a. The strips were run in a manifold on an ettan ipgphor 3 instrument. Its best to use the wire leads that come with your et232 or other leads with a rightangle style connector. The item you receive may be different from the actual item in the photo in the case of multiple quantity lots. The ettan daltsixsystem uses the same cassettes as the ettan dalttwelve electrophoresis system see fig 3. For detailed instructions on installation and usage of this software, refer to the ettan ipgphor 3 control software user manual.
Ettan daltsixlarge vertical electrophoresis system datafile 2d electrophoresis for second dimension 2d electrophoresis df80649002rev. Processing small tissue or cell samples using sample. Straight plugs can exert more lateral force on the jack inside the et232 and may cause damage if handled roughly. Match together the two spot maps using the decyder 2d software and create a pick list for all the spots corresponding to the cy 5 labeled cell surface proteins. Ief using ettan ipgphor 3 isoelectric focusing system and. Guidelines for ettan ipgphor with rehydration loadingief for. For use with equipment ettan ipgphor 3 isoelectric focusing unit. Ettan daltsix electrophoresis system user manual ge healthcare 80649249ac. The uninstall ettan ipgphor ii control software program will start. Prepare the ipgphor 3 system for sample loading and. Selective labelling of cellsurface proteins using cydye dige.
Ettan ipgphor isoelectric focusing system ipgphor function and description um 80641535 p2 control panel negative cathode electrode area positive anode electrode area cover pressure block sample application wells base 1. See the resources below for additional information. Inserting gels into the ettan dalt electrophoresis buffer tank. Its objective is to provide everything needed for successful operation. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3. Ettan ipgphor 3, ettan dalttwelve system image analysis. Speed and throughput with ettan ipgphor 3 ief system ettan ipgphor 3 ief system simplifies the handling of multiple samples and increases the speed and reproducibility of first dimension electrophoresis. Instrument components five strip holder lengths are available 7, 11, 18, and 24 cmone for each ipg. Divided into compartments with a platform in the middle, the horizontal electrophoresis systems completely submerge plated gel platforms in a buffer. Ettan ipgphor 3 isoelectric focusing unit 45002623 unit to run first.
Programmable functions include rehydration time, platform. The ettan dige system user manual recommends labeling. Protease inhibition using protease inhibitor mix sigma. Ge healthcare ettan daltsix user manual pdf download. Ettan ipgphor 3 operating instructions 28963551 ac 5 operation 5. The excellent first dimension separation ensures that spots in the second dimension are well separated fig 4. Precast dige gels and dige buffer kit were used for the seconddimension run in ettan dalttwelve electrophoresis unit according to the dige gel instruction. Read 2d electrophoresis principles and methods, ettan. Selective labelling of cellsurface proteins using cydye. For the inhibition of metalloproteases, add edta directly in an appropriate volume of extraction buffer or extract to give a final concentration of 5 mm edta in the reaction. Users guide see the online users guide for detailed information on using your product, including these features. Data file 28937442 aa 2d electrophoresis ipgbox and ipgbox kit.
Es3052 series, 52port gepoe layer 2 managed switch with 48 10 100 baset rj45 ports, and 4 gigabit sfp ports. Ipgphor 3 instrument user manual and ettan ipgphor 3 safety handbook prior to operation of the ettan. Pick cell surface proteins using the ettan spot handling station, extract from the gel plugs, and trypsinate using the ettan digester. Proteomic profiling of the epithelialmesenchymal transition using 2d dige. Ettan daltsixelectrophoresis unit are maintained by the combination of the builtin ceramic heat exchanger and buffer circulation pump with an external multitemp iii thermostatic circulator. If any part is missing, please contact your local ge healthcare sales of fice. Ipgphor manifold light 11002688 routine applications. Synchronized solutions for better 2d electrophoresis. Ettan ipgphor 3 control software user manual 11003459 ettan ipgphor 3 instrument user manual 11003458 ettan ipgphor cup loading manifold user manual 11003460 2d electrophoresis principles and methods 80642960 ettan dalttwelve system user manual 80647653. For the inhibition of metalloproteases, add edta directly in an appropriate volume of extraction buffer or extract to give a. Ettan ipgphor 3 isoelectric focusing system function and description 1 ettan ipgphor 3 instrument user manual 11003458 edition ab control panel the ettan ipgphor 3 is controlled by a 7key membrane keypad. Two 24cm immobilized precast gradient ipg strips immobilinetm drystrip gels, ph 4. These amounts will most likely vary depending on cell.
Equilibrate the focused ipg strips in sds equilibration. Ettan daltsixelectrophoresis system function and description in 2d electrophoresis, proteins are separated according to isoelectric point by isoelectric focusing, most reliably on immobiline drystrip immobilized ph gradient ipg gel strips using the ipgphor or multiphor ii ief systems. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3 isoelectric focusing system the ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, ease of use, speed, reproducibility, and high proteinloading capacity. Ettan ipgphor 3 isoelectric focusing unit 11003364 unit to run first dimension ief ipgphor manifold, complete 80649838 for the most stringent applications. Please read this entire manual to fully understand the safe. Uvc light kills germs without the use of chemicals. Et 2700et 2750 quick guide this guide includes basic information about your product and troubleshooting tips. Connect wirelessly from your smartphone, tablet, or computer. Data file 28937442 aa 2d electrophoresis ipgbox and. Read the instruction manual fully before starting up the um3b unit. Ettan ipgphor3 ief system 7cm strip protocols youtube. Ettan ipgphor 3 control software user manual 11003459.
The user can create, save, and edit protocols and also pause during a run to edit a protocol in real time. If a higher potency of protease inhibition is required, add protease inhibitor mix at a concentration of 2030. The manifold can accommodate all ipg strip sizes from 7 to 24 cm. Ge healthcare offers two systems for firstdimension separation. Equilibrate the focused ipg strips in sds equilibration buffer plus 1. The ettan ipgphor 3 control panel allows up to 10 user defined ief protocols, each with nine steps. Ettan ipgphor 3 control software generates professional graphical reports with a large user interface for programming and editing. Free shipping on filters available to us customers in the lower 48 states only uvc light destroys the dna of germs reducing their ability to reproduce. The total protein amount derived from 10 x10 6 chok1 cells was 920. The system components include the ettan daltsixelectrophoresis unit, gel caster, gradient maker, an eps 601 power. Data file 28937442 aa 2d electrophoresis ipgbox and ipgbox kit ipgbox.
The manifold is designed to handle ief and subsequent equilibration for up to 12 ipg strips. Ettan ipgphor ii manualzz owner guides and user manuals. Ettan ipgphor manifolds reduce handson time for running up to 12 ipg strips simultaneously. After ief, equilibrate the strips in two steps and place on top of large 26 x 20 cm 12. Protein concentrations in the different fractions were determined using the 2d quant kit. Pdf selective labelling of cellsurface proteins using. Imaging twodimensional protein gels stained with sypro ruby. Ettan ipgphor 3 manifold is an accessory for firstdimension ief of proteins on ipg strips. E3 system chart english, francais, espanol 365 kb adobe reader most olympus digital products are bundled with this program and it is typically installed automatically during software setup. The ettan dalt ii system is designed to handle multiple large seconddimension gels in a simple, ef. Page 1 ge healthcare ettan daltsix electrophoresis system user manual page 3 12 psig. All strips in a given run must be of the same length. Multiphor ii flatbed electrophoresis unit is another alternative. Amersham pharmacia biotech 3 ipgphor isoelectric focusing system user manual control panel the ipgphor is controlled by a 7key membrane keypad.